In general the rate of a chemical reaction increases with rising temperature. This also holds true when the reaction is catalysed by an enzyme - up to a point. The fact that enzymes are complicated proteins and tend to become unstable under certain conditions, complicates the matter. This Effect of Temperature on Enzymes Kit investigates these phenomena using the enzymes Amylase and Invertase. Suitable for advanced or tertiary level students. Experiments include:. Heat denaturation of Invertase. Heat denaturation of Amylase. Effect of incubation temperature on the activity of Amylase. The kit contains:. Potassium Sodium Tartarate. Sucrose. Sodium Hydroxide. Buffer pH7. Buffer pH6.5. Invertase 'Bioinvert'. Amylase Working Solution. 3,5-Dinitrosalicylic Acid. 1% Iodine Solution. Sufficient materials for 10 students/student groups.
